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Tep1 KN2 0 Mouse gene knockout kit via CRISPR non homology mediated
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Image Search Results
Journal: Parasites & Vectors
Article Title: csi-miR-96-5p delivered by Clonorchis sinensis extracellular vesicles promotes intrahepatic cholangiocarcinoma proliferation and migration via the ferroptosis-related PTEN/SLC7A11/GPX4 axis
doi: 10.1186/s13071-023-06075-7
Figure Lengend Snippet: PTEN inhibited ICC proliferation and migration via a ferroptosis mechanism. a Stable PTEN overexpression (PTEN-EXO) or CRISPR/Cas9-based knockout (PTEN-KO) HuCCT1 cell lines were established; the scale shown is 100 um. b Cell proliferation was measured by CCK-8 assay in vitro. c Cell proliferation was measured by tumor xenograft in vivo. d Cell migration was measured by transwell assay. e Cell GSH/GSSG radio assay. f Cell Fe 2+ assay. g Cell MDA assay. h , i Cell WB assay of SLC7A11 and GPX4. ICC , intrahepatic cholangiocarcinoma; WB , western blotting; GSH , glutathione; GSSG , GSH disulfide; MDA , malondialdehyde. * P < 0.05, ** P < 0.01
Article Snippet: To create stable cell lines, recombinant lentivirus (LV) vectors containing csi-miR-96-5p mimics (Shanghai Genchem Co., Ltd.), a PTEN overexpression plasmid (PTEN-EXO) (Sino Biological, China), and a
Techniques: Migration, Over Expression, CRISPR, Knock-Out, CCK-8 Assay, In Vitro, In Vivo, Transwell Assay, Multiple Displacement Amplification, Western Blot